Bhatt Garima, da Silva Rosa, Nachman Ronald J, Orchard Ian
Department of Biology, University of Toronto Mississauga, Mississauga, ON L5L 1C6, Canada.
Areawide Pest Management Research, Southern Plains Agricultural Research Center, U.S. Department of Agriculture, College Station, TX 77845, USA.
Peptides. 2014 Mar;53:148-58. doi: 10.1016/j.peptides.2013.04.009. Epub 2013 Apr 26.
The dramatic feeding-related activities of the Chagas' disease vector, Rhodnius prolixus are under the neurohormonal regulation of serotonin and various neuropeptides. One such family of neuropeptides, the insect kinins, possess diuretic, digestive and myotropic activities in many insects. In this study, we have cloned and examined the spatial expression of the R. prolixus kinin (Rhopr-kinin) transcript. In addition, in situ hybridization has been used to map the distribution of neurons expressing the kinin transcript. Physiological bioassays demonstrate the myostimulatory effects of selected Rhopr-kinin peptides and also illustrate the augmented responses of hindgut contractions to co-application of Rhopr-kinin and a R. prolixus diuretic hormone. Two synthetic kinin analogs have also been examined on the hindgut. These reveal interesting properties including a relatively irreversible effect on hindgut contractions and activity at very low concentrations.
恰加斯病病媒昆虫——南美锥蝽（Rhodnius prolixus）与进食相关的剧烈活动受血清素和多种神经肽的神经激素调节。其中一类神经肽家族，即昆虫激肽，在许多昆虫中具有利尿、消化和促肌作用。在本研究中，我们克隆并检测了南美锥蝽激肽（Rhopr-kinin）转录本的空间表达。此外，原位杂交已用于绘制表达激肽转录本的神经元分布。生理生物测定证明了所选Rhopr-kinin肽的促肌作用，还说明了后肠收缩对Rhopr-kinin与南美锥蝽利尿激素共同应用的增强反应。还在后肠上检测了两种合成激肽类似物。这些显示出有趣的特性，包括对后肠收缩的相对不可逆作用以及在极低浓度下的活性。
