Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada.
Insect Control and Cotton Disease Research, Southern Plains Agricultural Research Centre, U.S Department of Agriculture, College Station, TX, 77845, USA.
Insect Biochem Mol Biol. 2019 Nov;114:103223. doi: 10.1016/j.ibmb.2019.103223. Epub 2019 Aug 26.
In the Chagas disease vector Rhodnius prolixus, the kinin and CAPA family of neuropeptides are implicated in feeding and diuresis-related behaviours, with Rhopr-kinins stimulating contractions of the midgut, salivary glands, and hindgut, and RhoprCAPA-2 functioning as an anti-diuretic hormone. The current study examined the effects of kinin and CAPA neuropeptides and their analogs on feeding and diuresis, and on hindgut contractions and MT fluid secretion in R. prolixus. The biostable Aib-containing kinin analog 2139[Φ1]wp-2 was found to have antifeedant effects, and to be more potent than Rhopr-kinin 2 in stimulating hindgut contractions. The CAPA analog 2129-SP3[Φ3]wp-2 induced the intake of a larger blood meal, and increased the rate of post-prandial rapid diuresis. RhoprCAPA-2, but not its analog, potentiated hindgut contractions induced by Rhopr-kinin 2. Potentiation was observed with the CAPA analog on 5-HT-stimulated increases in frequency of hindgut contractions, whereas RhoprCAPA-2 inhibited this 5-HT-mediated stimulation. The CAPA analog induced hindgut contractions and prevented the inhibition induced by RhoprCAPA-2 on 5-HT-stimulated MT secretion. These results demonstrate novel interactions between Rhopr-kinin and RhoprCAPA-2 on the hindgut, possibly influencing post-feeding excretion. The kinin analog is a potent agonist of the kinin receptor, and the CAPA analog an antagonist of the CAPA receptor. The use of neuropeptide mimetics is a promising approach to vector control as they can disrupt behaviours, and the effects of these neuropeptide analogs highlight their value as lead compounds, given their ability to interfere with epidemiologically-relevant behaviours.
在恰加斯病传播媒介丽蝇 procoxus 中，激肽和 CAPA 家族神经肽参与摄食和利尿相关行为，丽蝇激肽刺激中肠、唾液腺和后肠收缩，丽蝇 CAPA-2 作为抗利尿激素。本研究检测了激肽和 CAPA 神经肽及其类似物对丽蝇 procoxus 摄食和利尿、后肠收缩和 MT 液分泌的影响。生物稳定的 Aib 含有激肽类似物 2139[Φ1]wp-2 具有抗摄食作用，比 Rhopr-kinin 2 更能刺激后肠收缩。CAPA 类似物 2129-SP3[Φ3]wp-2 诱导摄入更大的血餐，并增加餐后快速利尿的速度。RhoprCAPA-2，但不是其类似物，增强 Rhopr-kinin 2 诱导的后肠收缩。在 5-HT 刺激下，CAPA 类似物观察到后肠收缩频率增加的增效作用，而 RhoprCAPA-2 抑制了这种 5-HT 介导的刺激。CAPA 类似物诱导后肠收缩，并防止 RhoprCAPA-2 对 5-HT 刺激 MT 分泌的抑制作用。这些结果表明 Rhopr-kinin 和 RhoprCAPA-2 在后肠上存在新的相互作用，可能影响进食后的排泄。激肽类似物是激肽受体的有效激动剂，CAPA 类似物是 CAPA 受体的拮抗剂。神经肽类似物的使用是一种很有前途的控制媒介的方法，因为它们可以破坏行为，这些神经肽类似物的作用强调了它们作为先导化合物的价值，因为它们能够干扰与流行病学相关的行为。
