Departamento de Química, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.
Environ Pollut. 2013 Jul;178:403-10. doi: 10.1016/j.envpol.2013.03.016. Epub 2013 Apr 25.
The comet assay and micronucleous test were used to assess the genotoxicity of organic compounds associated with particulate material collected in the city of Córdoba, Argentina. Samples were collected on fiber glass filters and their organic extracts were analyzed by GC-MS. These extracts were used for the comet assay on human lymphocytes and for the MCN test with Tradescantia pallida. The concentrations of polycyclic aromatic hydrocarbons as well as some of their nitro derivates were higher during winter. Their composition suggested that their main emission sources were gasoline and diesel vehicles. We observed genotoxic effects of these organic extracts due to the presence of both direct and indirect acting mutagens. We found a good agreement between the two test systems employed, which encourages the further use of plant bioassays for air pollution monitoring, especially in developing countries, due to their flexibility, low cost and efficiency.
彗星试验和微核试验用于评估与阿根廷科尔多瓦市采集的颗粒物相关的有机化合物的遗传毒性。样品采集在玻璃纤维滤纸上，并用 GC-MS 对其有机提取物进行分析。这些提取物用于人淋巴细胞的彗星试验和 Tradescantia pallida 的微核试验。多环芳烃及其一些硝基衍生物的浓度在冬季较高。它们的组成表明其主要排放源是汽油和柴油车辆。由于存在直接和间接作用的诱变剂，我们观察到这些有机提取物的遗传毒性效应。我们发现所使用的两种测试系统之间具有良好的一致性，这鼓励进一步将植物生物测定法用于空气污染监测，特别是在发展中国家，因为它们具有灵活性、低成本和高效率。
