Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500, B.Cekmece, Istanbul, Turkey.
Bioprocess Biosyst Eng. 2013 Dec;36(12):1807-16. doi: 10.1007/s00449-013-0955-x. Epub 2013 Apr 27.
Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu(2+), Zn(2+), Cr(2+), Ni(2+)) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelated magnetic particles were used in the adsorption of invertase. The maximum invertase immobilization capacity of the PVIgMNP-Cu(2+) beads was observed to be 142.856 mg/g (invertase/PVIgMNP) at pH 5.0. The values of the maximum reaction rate (V max) and Michaelis-Menten constant (Km) were determined for the free and immobilized enzymes. The enzyme adsorption-desorption studies, pH effect on the adsorption efficiency, affinity of different metal ions, the kinetic parameters and storage stability of free and immobilized enzymes were evaluated.
聚乙烯亚胺（PVI）接枝的氧化铁纳米粒子（PVIgMNP）是通过将 PVI 的端粒接枝到氧化铁纳米粒子上制备的。不同的金属离子（Cu(2+)、Zn(2+)、Cr(2+)、Ni(2+)）离子螯合在聚乙烯亚胺接枝的氧化铁纳米粒子上，然后将金属螯合磁性颗粒用于转化酶的吸附。在 pH 5.0 时，观察到 PVIgMNP-Cu(2+)珠的最大转化酶固定化容量为 142.856 mg/g（转化酶/PVIgMNP）。测定了游离酶和固定化酶的最大反应速率（V max）和米氏常数（Km）的值。评估了酶的吸附-解吸研究、pH 对吸附效率的影响、不同金属离子的亲和力、动力学参数和游离酶和固定化酶的储存稳定性。
