Waifalkar P P, Parit S B, Chougale A D, Sahoo Subasa C, Patil P S, Patil P B
Thin Film Materials Laboratory, Department of Physics, Shivaji University Kolhapur, MS, India.
Department of Chemistry, The New College, Kolhapur, MS, India.
J Colloid Interface Sci. 2016 Nov 15;482:159-164. doi: 10.1016/j.jcis.2016.07.082. Epub 2016 Jul 30.
Industrially important invertase enzyme was immobilized on chitosan coated sol gel derived γ-Fe2O3 magnetic nanoparticles (MNPs) to enable it for repetitive use by magnetic separation. MNPs were characterized by X-ray diffraction (XRD), dynamic light scattering (DLS), field emission scanning electron microscope (FE-SEM), Fourier transform infrared (FTIR) spectrometer and magnetic measurements. FTIR studies confirmed successful immobilization of invertase on MNPs. The ability to convert sucrose into invert syrup was enhanced in immobilized invertase compared to that of free enzyme. Further it was found that invertase immobilized on MNPs (IIMNPs) were more stable at varying pH and temperature conditions. Magnetic separation technique was successfully employed for reuse of the IIMNPs for 20 times without significant loss of activity.
具有工业重要性的转化酶被固定在壳聚糖包覆的溶胶-凝胶衍生γ-Fe2O3磁性纳米颗粒（MNPs）上，以便通过磁分离实现其重复使用。通过X射线衍射（XRD）、动态光散射（DLS）、场发射扫描电子显微镜（FE-SEM）、傅里叶变换红外（FTIR）光谱仪和磁性测量对MNPs进行了表征。FTIR研究证实了转化酶成功固定在MNPs上。与游离酶相比，固定化转化酶将蔗糖转化为转化糖浆的能力有所增强。此外，还发现固定在MNPs上的转化酶（IIMNPs）在不同的pH和温度条件下更稳定。磁分离技术成功用于IIMNPs的重复使用20次，而活性没有明显损失。
