Leibniz-Institut für Polymerforschung Dresden e.V., P.O. Box 120 411, 01005 Dresden, Germany.
Anal Bioanal Chem. 2013 Jun;405(16):5421-30. doi: 10.1007/s00216-013-6967-1. Epub 2013 Apr 28.
The molecular imaging of paper cross sections containing the wet-strength additive poly(amidoamine)-epichlorohydrin (PAE) was effected by Fourier transform infrared (FT-IR) spectroscopic imaging. Thin cross sections of laboratory sheet samples were prepared and transferred onto CaF2 substrates. A laboratory sheet sample without PAE acted as a reference. Principal component analysis (PCA) was applied to identify and to reveal the distribution of PAE across the section. Differences in the loading plots of the fourth and fifth principal components for the sheets with and without PAE were found in the region of the amide I, amide II, and amine bands within a variance of 0.4-0.8%. The score images of the PCA reveal inhomogeneous distribution of PAE. Small areas of higher concentration of PAE occur across the cross section. The aim of this study was to demonstrate that FT-IR spectroscopic imaging provides spatially resolved quantitative information about the chemical composition of paper, which was successfully achieved.
采用傅里叶变换红外（FT-IR）光谱成像技术对含有湿强添加剂聚（酰胺-表氯醇）（PAE）的纸张横截面进行分子成像。制备实验室纸张样本的薄横截面并转移到 CaF2 衬底上。没有 PAE 的实验室纸张样本用作参比。应用主成分分析（PCA）来识别和揭示 PAE 在整个截面中的分布。在酰胺 I、酰胺 II 和胺带的范围内，发现有无 PAE 的纸张的第四和第五主成分加载图之间存在差异，方差为 0.4-0.8%。PCA 的得分图像显示出 PAE 的不均匀分布。PAE 的浓度较高的小区域出现在整个横截面上。本研究的目的是证明 FT-IR 光谱成像技术可提供有关纸张化学成分的空间分辨定量信息，这一目标已成功实现。
