Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Anal Bioanal Chem. 2013 Jun;405(16):5539-47. doi: 10.1007/s00216-013-6979-x. Epub 2013 Apr 28.
We report here on differential linear scan voltammetry, DLSV, that combines the working principles of linear scan voltammetry, LSV, and the numerous existing pulsed voltammetry techniques. DLSV preserves the information from continuous interrogation in voltage and high accuracy that LSV provides about electrochemical processes, and the much better sensitivity of differential pulsed techniques. DLSV also minimizes the background current compared to both LSV and pulsed voltammetry. An early version of DLSV, derivative stationary electrode polarography, DSEP, had been proposed in the 1960s but soon abandoned in favor of the emerging differential pulsed techniques. Relative to DSEP, DLSV takes advantage of the flexibility of discrete smoothing differentiation that was not available to early investigators. Also, DSEP had been explored in pure solutions and with reversible electrochemical reactions. DLSV is tested in this work in more challenging experimental contexts: the measurement of oxygen with a carbon fiber microelectrode in buffer, and with a gold microdisc electrode exposed to a live biological preparation. This work compares the analytical performance of DLSV and square wave voltammetry, the most popular pulsed voltammetry technique.
我们在此报告差分线性扫描伏安法（DLSV），它结合了线性扫描伏安法（LSV）和众多现有的脉冲伏安技术的工作原理。DLSV 保留了 LSV 提供的关于电化学过程的连续电压询问和高精度信息，以及差分脉冲技术的更高灵敏度。与 LSV 和脉冲伏安法相比，DLSV 还能最小化背景电流。DLSV 的早期版本，即导数静止电极极谱法（DSEP），在 20 世纪 60 年代就已提出，但很快就被新兴的差分脉冲技术所取代。与 DSEP 相比，DLSV 利用了离散平滑微分的灵活性，这在早期研究中是无法实现的。此外，DSEP 曾在纯溶液和具有可逆电化学反应的体系中进行过探索。在这项工作中，DLSV 在更具挑战性的实验环境中进行了测试：在缓冲液中使用碳纤维微电极测量氧气，以及使用金微盘电极暴露于活体生物制剂中。本工作比较了 DLSV 和方波伏安法（最流行的脉冲伏安技术）的分析性能。
