Department of Hospitality Management, Chu Hwa University of Medical Technology, 91 Wenhua 1st Street, Zender, Tainan, 717 Taiwan.
Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, 300 Taiwan.
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2351-2355. doi: 10.1099/ijs.0.051227-0. Epub 2013 Apr 26.
Two novel yeast species, Tetrapisispora taiwanensis sp. nov. and Tetrapisispora pingtungensis sp. nov., belonging to the Saccharomycetaceae within the Saccharomycetales, are proposed to accommodate six strains isolated from samples of Taiwanese forest soil between 2005 and 2010. Sequence analysis of the internal transcribed spacer (ITS) and the D1/D2 domains of the large-subunit (LSU) rRNA gene revealed that the two species are phylogenetically closely related to species of the genus Tetrapisispora. Moreover, the assimilation spectrum of carbon and nitrogen sources and morphological characteristics are very similar to those of other Tetrapisispora species. The molecular, morphological and physiological characteristics described above indicate that these two species should be classified as members of the genus Tetrapisispora. The two species can be differentiated from each other and from other Tetrapisispora species based on their LSU D1/D2 rRNA gene and ITS sequences. Thus, the two species could be regarded as novel species of the genus Tetrapisispora, and the names Tetrapisispora taiwanensis sp. nov. (type strain SJ6S04(T) =CBS 10586(T) =NBRC 102652(T) =BCRC 23090(T)) and Tetrapisispora pingtungensis sp. nov. (type strain NC2S06(T) =CBS 12780(T) =BCRC 23409(T)) are proposed. The type strains of the two species were isolated from forest soil in Leinhuatsu Park, Nantou, in 2006 and from Hungchun, Pingtung, in 2009, respectively.
两种新型酵母种，即 Tetrapisispora taiwanensis sp. nov. 和 Tetrapisispora pingtungensis sp. nov.，隶属于 Saccharomycetaceae 科 Saccharomycetales 目，是为了容纳六株分别于 2005 年至 2010 年期间从台湾森林土壤样本中分离出来的菌株而提出的。对内转录间隔区（ITS）和大亚基（LSU）rRNA 基因的 D1/D2 结构域的序列分析表明，这两个种在系统发育上与 Tetrapisispora 属的种密切相关。此外，碳和氮源同化谱以及形态特征与其他 Tetrapisispora 种非常相似。上述分子、形态和生理特征表明，这两个种应被归类为 Tetrapisispora 属的成员。这两个种可以根据其 LSU D1/D2 rRNA 基因和 ITS 序列彼此以及与其他 Tetrapisispora 种区分开来。因此，这两个种可以被视为 Tetrapisispora 属的新型种，名称为 Tetrapisispora taiwanensis sp. nov.（模式株 SJ6S04(T) =CBS 10586(T) =NBRC 102652(T) =BCRC 23090(T)）和 Tetrapisispora pingtungensis sp. nov.（模式株 NC2S06(T) =CBS 12780(T) =BCRC 23409(T)）。这两个种的模式株分别于 2006 年从南投县鲤鱼潭公园的森林土壤中和 2009 年从屏东县恒春的森林土壤中分离得到。
