Krizman David B, Burrows Jon
Oncoplex Diagnostics, Rockville, MD, USA.
Methods Mol Biol. 2013;1002:85-92. doi: 10.1007/978-1-62703-360-2_7.
Application of mass spectrometry to proteomic analysis of tissue is a highly desirable approach to discovery of disease biomarkers due to a direct correlation of findings to tissue/disease histology and in many respects obviating the need for model systems of disease. Both frozen and formalin-fixed, paraffin-embedded (FFPE) tissue can be interrogated; however, worldwide access to vastly larger numbers of highly characterized FFPE tissue collections derived from both human and model organisms makes this form of tissue more advantageous. Here, an approach to large-scale, global proteomic analysis of FFPE tissue is described that can be employed to discover differentially expressed proteins between different histological tissue types and thus discover novel protein biomarkers of disease.
将质谱技术应用于组织的蛋白质组学分析，是发现疾病生物标志物的一种非常理想的方法，因为研究结果与组织/疾病组织学直接相关，并且在许多方面无需疾病模型系统。冷冻组织和福尔马林固定、石蜡包埋（FFPE）组织均可进行检测；然而，在全球范围内，能够获取大量来自人类和模式生物的特征明确的FFPE组织样本，使得这种组织形式更具优势。本文描述了一种对FFPE组织进行大规模、全面蛋白质组学分析的方法，该方法可用于发现不同组织学类型之间差异表达的蛋白质，从而发现新的疾病蛋白质生物标志物。
