Hood Brian L, Conrads Thomas P, Veenstra Timothy D
Laboratory of Proteomics and Analytical Technologies at SAIC-Frederick, Inc., MD 21702, USA.
Brief Funct Genomic Proteomic. 2006 Jun;5(2):169-75. doi: 10.1093/bfgp/ell017. Epub 2006 May 10.
Biofluid detectable biomarkers that originate at the site of diseased tissues would be advantageous, in that, they may provide mechanistic information concerning the manifestation and progression of the disease. Unfortunately, tissue biopsies are precious samples that can generally be acquired in small amounts due to the invasive nature of the sample collection. One of the foundations of pathological diagnosis for decades has been from formalin-fixed paraffin-embedded (FFPE) tissues, of which a vast archive exists worldwide. These tissues have also been widely used for immunohistochemistry and in situ hybridization studies examining for expression of specific proteins or transcripts. Unfortunately, the ability to analyse FFPE tissues using mass spectrometry (MS) has been essentially non-existent until recently. In this review, methods that allow the extraction of peptides from FFPE tissues and their proteomic analysis using MS are described. The ability to identify the proteins extracted from FFPE tissues allows comparative analyses that enable the potential discovery of novel biomarkers at the site of the diseased tissue.
起源于病变组织部位的生物流体可检测生物标志物将具有优势，因为它们可能提供有关疾病表现和进展的机制信息。不幸的是，组织活检样本很珍贵，由于样本采集具有侵入性，通常只能获取少量样本。几十年来，病理诊断的基础之一一直是来自福尔马林固定石蜡包埋（FFPE）组织，全球存在大量此类存档组织。这些组织也被广泛用于免疫组织化学和原位杂交研究，以检测特定蛋白质或转录本的表达。不幸的是，直到最近，利用质谱（MS）分析FFPE组织的能力基本上还不存在。在这篇综述中，描述了从FFPE组织中提取肽段并使用MS进行蛋白质组学分析的方法。识别从FFPE组织中提取的蛋白质的能力允许进行比较分析，从而有可能在病变组织部位发现新的生物标志物。
