Chemokine-dependent signaling pathways in the peripheral nervous system.

Chemokines and their G-protein-coupled receptors play important roles in development, homeostasis, and the innate and adaptive immune response. Pathologic chemokine signaling pathways in the peripheral nervous system can be studied in peripheral nerves using human in vitro models of the blood-nerve barrier (BNB) and a reliable model of acute peripheral nerve inflammation called severe murine experimental autoimmune neuritis (EAN). This chapter describes a flow-dependent human leukocyte-BNB trafficking assay and the reliable induction of EAN in female SJL/J mice as tools to study pro-inflammatory chemokine-dependent signaling in peripheral nerves.