施万细胞种植于去细胞神经移植物可改善功能恢复。
Schwann cells seeded in acellular nerve grafts improve functional recovery.
机构信息
Department of Biomedical Engineering, Washington University, Campus Box 1097, One Brookings Drive, St. Louis, Missouri, 63130, USA.
出版信息
Muscle Nerve. 2014 Feb;49(2):267-76. doi: 10.1002/mus.23885. Epub 2013 Nov 22.
INTRODUCTION
This study evaluated whether Schwann cells (SCs) from different nerve sources transplanted into cold-preserved acellular nerve grafts (CP-ANGs) would improve functional regeneration compared with nerve isografts.
METHODS
SCs isolated and expanded from motor and sensory branches of rat femoral and sciatic nerves were seeded into 14mm CP-ANGs. Growth factor expression, axonal regeneration, and functional recovery were evaluated in a 14-mm rat sciatic injury model and compared with isografts.
RESULTS
At 14 days, motor or sensory-derived SCs increased expression of growth factors in CP-ANGs versus isografts. After 42 days, histomorphometric analysis found CP-ANGs with SCs and isografts had similar numbers of regenerating nerve fibers. At 84 days, muscle force generation was similar for CP-ANGs with SCs and isografts. SC source did not affect nerve fiber counts or muscle force generation.
CONCLUSIONS
SCs transplanted into CP-ANGs increase functional regeneration to isograft levels; however SC nerve source did not have an effect.
简介
本研究评估了源自不同神经来源的施万细胞（SCs）移植到冷保存去细胞神经移植物（CP-ANGs）中是否比神经同种异体移植物更能促进功能再生。
方法
从大鼠股神经和坐骨神经的运动和感觉分支中分离和扩增 SCs，将其接种到 14mm 的 CP-ANGs 中。在 14mm 大鼠坐骨神经损伤模型中评估生长因子表达、轴突再生和功能恢复，并与同种异体移植物进行比较。
结果
在 14 天时，源自运动或感觉的 SCs 增加了 CP-ANGs 中生长因子的表达，而不是同种异体移植物。42 天后，组织形态计量学分析发现 CP-ANGs 与 SCs 和同种异体移植物具有相似数量的再生神经纤维。84 天时，CP-ANGs 与 SCs 和同种异体移植物的肌肉力量生成相似。SC 来源对神经纤维计数或肌肉力量生成没有影响。
结论
移植到 CP-ANGs 中的 SCs 增加了功能再生至同种异体移植物的水平；然而，SC 神经来源没有影响。
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