State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Appl Biochem Biotechnol. 2013 Jun;170(4):951-61. doi: 10.1007/s12010-013-0234-y. Epub 2013 Apr 30.
Recombinant Escherichia coli cells expressing phenol hydroxylase (designated as strain PH(IND)) were used to synthesize chloro-substituted indigoids by the transformation of indoles. The optimal conditions for the biotransformation of 4- and 7-chloroindole were determined by response surface methodology. Biotransformation kinetic assays revealed that strain PH(IND) showed high catalytic efficiency for 4- and 7-chloroindole. The formation rate of 7,7'-dichloroindigo (1.35 unit/mg cell dry weight) by strain PH(IND) was 1.14-fold higher than that of 4,4'-dichloroindigo. The intermediates of 7-chloroindole biotransformation were identified by high-performance liquid chromatography-mass spectroscopy, and the biotransformation mechanism was also proposed. These results suggested that there was a potential application of strain PH(IND) in the biotransformation of chloro-substituted indoles to valuable indigoids.
表达苯酚羟化酶的重组大肠杆菌细胞（命名为 PH(IND) 菌株）被用于通过吲哚的转化来合成氯取代的靛蓝。通过响应面法确定了 4-氯吲哚和 7-氯吲哚转化的最佳条件。生物转化动力学分析表明，PH(IND) 菌株对 4-氯吲哚和 7-氯吲哚具有很高的催化效率。PH(IND) 菌株形成 7,7'-二氯靛蓝（1.35 单位/毫克细胞干重）的速率比 4,4'-二氯靛蓝高 1.14 倍。通过高效液相色谱-质谱鉴定了 7-氯吲哚生物转化的中间体，并提出了生物转化机制。这些结果表明，PH(IND) 菌株在氯取代吲哚向有价值的靛蓝的生物转化方面具有潜在的应用。
