State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Appl Microbiol Biotechnol. 2013 Jun;97(11):5097-103. doi: 10.1007/s00253-012-4301-z. Epub 2012 Aug 2.
Phenol hydroxylase gene engineered microorganism (PHIND) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and (1)H NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 μM/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation.
酚羟化酶基因工程菌（PHIND）被用于通过连续羟化反应从苯和甲苯合成儿茶酚。HPLC-MS 和 (1)H NMR 分析证明，生物转化的产物是相应的儿茶酚，通过中间产物苯酚的生成。这表明甲苯氧化的主要产物是邻甲酚和对甲酚。间甲酚生物转化的主要产物是 3-甲基儿茶酚。儿茶酚（25 μM/min/g 细胞干重）的生成速率比甲基儿茶酚高 1.43 倍。这表明酚羟化酶可以成功地用于通过连续羟化将苯和甲苯转化为儿茶酚。
