The State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, People's Republic of China.
J Biochem Mol Toxicol. 2013 May;27(5):279-85. doi: 10.1002/jbt.21488. Epub 2013 Apr 26.
The toxic interaction between 2-naphthylamine (2-NA) and herring sperm deoxyribonucleic acid (hs-DNA) has been thoroughly investigated by UV absorption, fluorescence, and circular dichroism (CD) spectroscopic methods. UV absorption result indicates that 2-NA may intercalate into the stack base pairs of DNA during the toxic interaction of 2-NA with DNA. A fluorescence quenching study shows that DNA quenches the intrinsic fluorescence of 2-NA via a static pathway. The studies on effects of ionic strength and anionic quenching rule out electrostatic and groove bindings as the dominant binding modes. Further studies on denatured DNA fluorescence quenching and thermal melting studies confirm that the dominant binding mode of 2-NA-DNA is intercalative binding. A CD spectral study shows that the binding interaction of 2-NA with DNA leads to the disorganization of the neat double-helical structure of hs-DNA.
2-萘胺（2-NA）与鲱精 DNA（hs-DNA）之间的毒性相互作用已通过紫外吸收、荧光和圆二色性（CD）光谱方法进行了深入研究。紫外吸收结果表明，在 2-NA 与 DNA 的毒性相互作用过程中，2-NA 可能插入 DNA 的堆叠碱基对中。荧光猝灭研究表明，DNA 通过静态途径猝灭 2-NA 的本征荧光。离子强度和阴离子猝灭效应的研究排除了静电和沟结合作为主要的结合模式。对变性 DNA 荧光猝灭和热融研究的进一步研究证实，2-NA-DNA 的主要结合模式是嵌入结合。CD 光谱研究表明，2-NA 与 DNA 的结合相互作用导致 hs-DNA 规整的双螺旋结构的解组织。
