Harasawa R, Koshimizu K, Uemori T, Takeda O, Asada K, Kato I
Division of Animal Research, Faculty of Medicine, University of Tokyo.
Microbiol Immunol. 1990;34(4):393-5. doi: 10.1111/j.1348-0421.1990.tb01020.x.
In vitro DNA amplification by polymerase chain reaction was examined to detect Mycoplasma pulmonis. A pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of M. pulmonis DNA. Amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. This system detected cellular DNA of M. pulmonis but not M. arthritidis or M. neurolyticum, and thus appears to be useful for M. pulmonis diagnosis.
