Kunita S, Terada E, Goto K, Kagiyama N
Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Sagamihara-shi, Japan.
Jikken Dobutsu. 1990 Jan;39(1):103-7. doi: 10.1538/expanim1978.39.1_103.
Detection of Mycoplasma pulmonis was examined by using the polymerase chain reaction (PCR) for amplifying a specific DNA sequence. In gel electrophoresis which was conducted to detect the amplified products, only 1 pg of M. pulmonis DNA could be detected following 30 cycles of amplification, while no amplified product was detected even from 1 microgram of M. arthritidis or M. neurolyticum DNA. Furthermore, 10 colony-forming units of M. pulmonis could be detected by direct amplification from the mycoplasma suspension. These results suggest the usefulness of the PCR as a highly sensitive, specific, and rapid method for direct detection of M. pulmonis.
