Department of Dermatology, Seoul National University College of Medicine, Medical Research Center, Seoul National University, Medical Research Center, Seoul National University, Seoul, Korea.
J Cell Physiol. 2013 Nov;228(11):2243-51. doi: 10.1002/jcp.24393.
ABO blood group is determined by carbohydrate antigens, called ABH antigens. It has been known that the change of carbohydrate antigen expression, including ABH antigens, has correlation with the tumor metastasis and survival; however, the exact mechanism remains to be elucidated. ABH antigens are expressed not only in blood cells but also in several tissues. In epidermis, ABH antigen is expressed in the uppermost spinous and granular layer. We investigated the role of ABH antigens on the cell migration of HaCaT keratinocytes, which express B antigen. Knock-down of B antigen expression by small interference RNA of FUT1 inhibited HaCaT cell migration. At that time, we found that lamellipodia and actin fiber were also reduced by knock-down of B antigen expression. The transcription of cdc42, a kind of Rho GTPase which plays a key role in actin polymerization, was reduced by down-regulated B antigen expression. Furthermore, the reduced B antigen expression also inhibited the interaction of cdc42 and N-WASP. Collectively, our data provide a clue how ABH antigens regulate the cell migration mechanism.
ABO 血型由碳水化合物抗原决定，称为 ABH 抗原。已知碳水化合物抗原表达的改变，包括 ABH 抗原，与肿瘤转移和存活有关；然而，确切的机制仍有待阐明。ABH 抗原不仅在血细胞中表达，也在几种组织中表达。在表皮中，ABH 抗原在上部棘层和颗粒层表达。我们研究了 ABH 抗原在表达 B 抗原的 HaCaT 角质形成细胞迁移中的作用。通过 FUT1 的小干扰 RNA 敲低 B 抗原表达抑制了 HaCaT 细胞的迁移。当时，我们发现 B 抗原表达的敲低也减少了片状伪足和肌动蛋白纤维。CDC42 的转录，一种在肌动蛋白聚合中起关键作用的 Rho GTPase，也被下调的 B 抗原表达所减少。此外，下调 B 抗原表达也抑制了 cdc42 和 N-WASP 的相互作用。总之，我们的数据提供了一个线索，说明 ABH 抗原如何调节细胞迁移机制。
