Narita Shintaro, Obara Takashi, Ishikawa Namiko, Suzuki Yoshihisa, Habuchi Tomonori
Department of Urology, Akita University School of Medicine, Akita, Japan.
Department of Urology, Akita University School of Medicine, Akita, Japan; Department of Urology, Akita Red Cross Hospital, Akita, Japan.
Sex Med. 2021 Feb;9(1):100308. doi: 10.1016/j.esxm.2020.100308. Epub 2021 Jan 12.
Neuroprotection and neuroregeneration of cavernous nerve plexus by biological/bioengineering solutions may have the potential to maintain erectile function.
We evaluated the efficacy of a newly developed artificial nerve sheet using freeze-dried alginate (ALG) with polyglycolic acid (PGA) mesh in a rat model.
Bilateral cavernous nerves of male rats were excised to make an approximately 2 mm gap. A piece of the sponge-like freeze-dried sheet created by covalent cross-linking of ALG gel combined with PGA mesh was placed over the gap to cover each stump without any neural anastomosis. We compared erectile functions in the ALG groups with those in the sham group and the bilateral nerve excision group (n = 12, each).
Main outcome measure was a rat model with cavernous nerve excision.
All rats in the sham group had erection at 63 or 64 days, and mating behavior was confirmed in 10 rats (83.3%) of the sham group at 56 to 62 days. No erection and mating behavior was observed in the excision group. Ten of the 12 (83.3%) rats in the ALG group had a mating behavior and an erection, and the rates of erection and mating behavior were significantly higher in the ALG group than those in the excision group (P < .01, P < .01, respectively). Using a retrograde FluoroGold, the rate of FluoroGold positive pelvic ganglia proximal to the gap at 61 or 62 days was significantly higher in the ALG group than that in the excision group (P = .014).
The results of our animal study have demonstrated that simply filling the cavernous nerve gap using the non-tubular artificial nerve sheets made of ALG with PGA mesh restored erectile function after cavernous nerve excision. Narita S, Obara T, Ishikawa N, et al. Cavernous Branched Nerve Regeneration Using Non-Tubular Artificial Nerve Sheets Using Freeze-Dried Alginate Gel Combined With Polyglycolic Acid Mesh in a Rat Model. Sex Med 2021;9:100308.
采用生物/生物工程方法对海绵体神经丛进行神经保护和神经再生可能具有维持勃起功能的潜力。
我们在大鼠模型中评估了一种新开发的使用冻干海藻酸盐（ALG）与聚乙醇酸（PGA）网片制成的人工神经片的疗效。
切除雄性大鼠双侧海绵体神经，造成约2毫米的间隙。将一块由ALG凝胶与PGA网片共价交联制成的海绵状冻干片置于间隙上，覆盖每个残端，不进行任何神经吻合。我们比较了ALG组与假手术组和双侧神经切除组（每组n = 12）的勃起功能。
主要观察指标是海绵体神经切除的大鼠模型。
假手术组所有大鼠在63或64天时均有勃起，56至62天时假手术组10只大鼠（83.3%）出现交配行为。切除组未观察到勃起和交配行为。ALG组12只大鼠中有10只（83.3%）出现交配行为和勃起，ALG组的勃起率和交配行为发生率显著高于切除组（分别为P <.01，P <.01）。使用逆行荧光金标记法，在61或62天时，ALG组间隙近端荧光金阳性盆腔神经节的比例显著高于切除组（P = 0.014）。
我们的动物研究结果表明，使用由ALG与PGA网片制成的非管状人工神经片简单填充海绵体神经间隙，可在海绵体神经切除后恢复勃起功能。成田S、小原T、石川N等。大鼠模型中使用冻干海藻酸盐凝胶与聚乙醇酸网片制成的非管状人工神经片进行海绵体分支神经再生。性医学2021；9：100308。
