1. Department of Biology, Faculty of Science, Tarbiat Moallem University of Tehran, Tehran, Iran.
Cell J. 2012 Spring;14(1):47-52. Epub 2012 Jun 13.
Medicinal plants are widely used throughout the world. Since these plants are known to have minimal side effects, many people embrace them. The golpar plant, scientifically known as Heracleum persicum (H. persicum), is a common Asian and Iranian medicinal plant. The use of golpar is recommended in traditional medicine as a contraceptive medication for females; however, no scientifically documented evidence has been reported. This study investigates the effects of the golpar plant on ovarian tissue and folliculogenesis.
In this experimental study, H. persicum hydroalcoholic extract (HPHE) was used at 400 mg/kg and 1600 mg/kg doses. Adult female rats were divided into three groups: control, sham, and experimental(I, II). The control group did not receive any injection, the sham group received saline solution, and the experimental group received IP injections of HPHE for 21 days, once every other day, during the sexual cycle. At the end of the injection period, ovarian samples were harvested for histological studies. The FSH assay was performed according to the chemiluminescence immunoassay (CLIA) method. Data were statistically analyzed by the Instat3 program and one-way ANOVA. A p value of <0.05 was considered significant.
In the experimental group the numbers of primordial and primary follicles increased (p <0.001), while the number of preantral and antral follicles decreased (p <0.01). The atretic follicles decreased in the experimental group, but this decrease was not significant. There was no statistical difference in FSH concentration when compared with the control group.
This report gives primary information on the in vivo effects of the HPHE on the ovarian follicles of the female Wistar rat. The results suggest that administration of HPHE may have inhibitory effects on folliculogenesis and cause infertility in females.
药用植物在世界各地被广泛使用。由于这些植物被认为副作用极小，许多人都喜欢使用它们。Golpar 植物，学名为 Heracleum persicum（H. persicum），是一种常见的亚洲和伊朗药用植物。传统医学中推荐使用 golpar 作为女性避孕药；然而，目前还没有科学记录的证据表明其有效性。本研究旨在探讨 golpar 植物对卵巢组织和卵泡发生的影响。
在这项实验研究中，使用了 H. persicum 水醇提取物（HPHE），剂量分别为 400mg/kg 和 1600mg/kg。成年雌性大鼠被分为三组：对照组、假手术组和实验组（I、II）。对照组未接受任何注射，假手术组给予生理盐水，实验组则在性周期内每隔一天接受 IP 注射 HPHE，共 21 天。在注射期结束时，采集卵巢样本进行组织学研究。根据化学发光免疫分析（CLIA）法进行 FSH 测定。数据采用 Instat3 程序和单因素方差分析进行统计分析。p 值<0.05 被认为具有统计学意义。
实验组原始卵泡和初级卵泡数量增加（p<0.001），而前腔卵泡和腔卵泡数量减少（p<0.01）。实验组闭锁卵泡数量减少，但差异无统计学意义。与对照组相比，FSH 浓度无统计学差异。
本报告提供了 HPHE 对雌性 Wistar 大鼠卵巢卵泡体内作用的初步信息。结果表明，HPHE 的给药可能对卵泡发生具有抑制作用，并导致女性不孕。
