School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
J Biomed Nanotechnol. 2013 Jan;9(1):115-23. doi: 10.1166/jbn.2013.1476.
Synthesis of nanoparticles with desired size/morphology has enormous importance, especially in the compelling field of nanotechnology. In this case, a novel kind of raspberry-type hybrid nanoparticles was prepared by hybridization of chitosan (CS) with thioethyl pendant ligand (TPL) modified Au nanoparticles (Au-TPL@CS NPs). Such method was based on ionic gelation using sodium tripolyphosphate as a counterion. The blood compatibility of Au-TPL@CS NPs was characterized by coagulation tests, plasma recalcification time, hemolysis assay, morphological changes of red blood cells (RBCs) and complement activation in vitro. The results showed that Au-TPL@CS NPs exhibited good blood compatibility. The possible underlying mechanism was also present. Finally, the direct electron transfer reactivity of the Hemoglobin/Au-TPL@CS NPs/multi-walled carbon nanotubes/glassy carbon electrode was investigated with cyclic voltammetry measurements. The biosensor exhibited a good electrocatalytic activity to the reduction of H2O2. Such new type of Au-TPL@CS NPs provides a promising platform of biological system for early illness detection and treatment in future.
具有所需尺寸/形态的纳米粒子的合成具有重要意义，尤其是在引人注目的纳米技术领域。在这种情况下，通过壳聚糖（CS）与硫乙基侧链配体（TPL）修饰的金纳米粒子（Au-TPL@CS NPs）的杂交，制备了一种新型覆盆子型杂化纳米粒子。该方法基于使用三聚磷酸钠作为抗衡离子的离子凝胶化。通过凝血试验、血浆再钙化时间、溶血试验、体外红细胞（RBC）形态变化和补体激活来表征 Au-TPL@CS NPs 的血液相容性。结果表明，Au-TPL@CS NPs 具有良好的血液相容性。还提出了可能的潜在机制。最后，通过循环伏安法测量研究了血红蛋白/Au-TPL@CS NPs/多壁碳纳米管/玻碳电极的直接电子转移反应性。该生物传感器对 H2O2 的还原表现出良好的电催化活性。这种新型的 Au-TPL@CS NPs 为未来的早期疾病检测和治疗提供了一个有前途的生物系统平台。
