Key Education Ministry Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Bioprocess Biosyst Eng. 2011 Nov;34(9):1143-50. doi: 10.1007/s00449-011-0565-4. Epub 2011 Jul 1.
In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO(2)-MWNTs-CS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet-vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 μM to 1.2 mM with a detection limit of 0.1 μM glucose (S/N = 3). In addition, this work indicated that TiO(2)-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors.
本文制备了一种新型葡萄糖生物传感器。首先，在 TiO(2)-MWNTs-CS 复合修饰的玻碳电极上电沉积普鲁士蓝（PB），然后将聚二烯丙基二甲基氯化铵（PDDA）功能化的金纳米粒子（PDDA-Au）吸附在 PB 膜上。最后，带负电荷的葡萄糖氧化酶（GOD）通过静电自组装到带正电荷的 PDDA-Au 上。通过循环伏安法（CV）和安培法分别研究了修饰电极的电化学性能。此外，还通过扫描电子显微镜对所制备的生物传感器的逐步制备过程进行了表征。通过紫外可见吸收光谱和透射电子显微镜对 PDDA-Au 纳米粒子进行了表征。在最佳条件下，所制备的生物传感器对葡萄糖具有良好的响应性能，线性范围为 6 μM 至 1.2 mM，检测限为 0.1 μM 葡萄糖（S/N = 3）。此外，这项工作表明 TiO(2)-MWNTs-CS 复合材料和 PDDA-Au 纳米粒子在构建生物传感器方面具有很大的潜力。
