Chen Jun, Liu Jia-Jian, Lu Li, Yang Guo-Zhu, Lu Xing-Yan, Huang Jian, Li Qing-Nan
School of Life Science and Biopharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhong Yao Cai. 2012 Nov;35(11):1820-4.
To study the influence of Plantaginis Semen on cell proliferation, differentiation and function of rat osteoblasts, and investigate the regulation effects of rat osteoblast epithelial sodium channel (ENaC) on bone formation.
The animal serum was prepared by serum pharmacology means. The cells were got by separating and inducing the SD neonatal rat's skull bone. Cell proliferation and differentiation were evaluated by CCK-8 assay kit and AKP assay kit respectively. Regulation effects on mRNA expression of ENaC and osteogenesis gene were investigated by semi-quantitative PCR.
Plantaginis Semen stimulated the osteoblasts proliferation and differentiation,the difference between treatment group and control group had statistical significance (P < 0.01) in a dose-dependent manner. The effects of Plantaginis Semen serum on alpha-ENaC gene expression paralleled those on osteogenic gene (OC, ALP, OP) expression level.
Plantaginis Semen stimulates proliferation, differentiation and the mRNA expression of ENaC and osteogenesis gene in rat osteoblasts. Our results suggest that ENaC participate in the effects of Plantaginis Semen serum on osteoblast bone formation. Regulation of ENaC channel expression and function may provide a new clue for research on treatment of osteoporosis with traditional Chinese medicine.
研究车前子对大鼠成骨细胞增殖、分化及功能的影响，探讨大鼠成骨细胞上皮钠通道（ENaC）对骨形成的调控作用。
采用血清药理学方法制备含药血清。分离并诱导SD新生大鼠颅骨获取细胞。分别用CCK-8试剂盒和碱性磷酸酶（AKP）试剂盒评价细胞增殖和分化情况。采用半定量PCR法研究对ENaC及成骨相关基因mRNA表达的调控作用。
车前子可促进成骨细胞增殖和分化，治疗组与对照组相比差异有统计学意义（P<0.01），且呈剂量依赖性。车前子含药血清对α-ENaC基因表达的影响与对成骨基因（OC、ALP、OP）表达水平的影响相似。
车前子可促进大鼠成骨细胞增殖、分化及ENaC和成骨相关基因的mRNA表达。结果提示ENaC参与了车前子含药血清对成骨细胞骨形成的作用。调控ENaC通道的表达及功能可能为中药治疗骨质疏松症的研究提供新线索。
