Zhou Tian-Lin, Jing Ming-Bo, Li Dong-Bo, Jiao Fei
University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu Province, College of Life Science and Technology, Longdong University, Qingyang 745000, China.
Zhong Yao Cai. 2012 Oct;35(10):1668-74.
To optimize the technology of enzymolysis extraction of total anthraquinones.
Used the total anthraquinone concentration extracted from rhubarb (Rheum palmatum, L) via enzymolysis as experimental assess index, effect of enzyme dose, enzyme solution temperature, enzyme solution time and pH on total anthraquinone extraction were investigated respectively based on single factor variance analysis. Furthermore, its extraction process was optimized based on response surface methodology.
The yield of total anthraquinones demonstrated the top (3.1730 +/- 0.1023 mg/L) while the enzyme dose was up to 0.12 g, besides, for the enzyme solution time, the total anthraquinones yield exhibited a trend of increase with the increase of enzyme solution time and the top concentration of total anthraquinones was up to 2.1410 +/- 0.1252 mg/L while it was 48 h; Moreover, the top field of total anthraquinones were up to 2.2777 +/- 0.2133 mg/L and 4.1360 +/- 0.3877 mg/L while the pH and enzyme solution temperature were up to 4.8 and 50 degrees C in respectively. Finally, the most optimum enzymolysis extraction processing technology via response surface methodology was as follows: pH: 4.59, enzyme dose: 0.1 g as well as enzyme solution temperature: 45.86 degrees C.
The condition of extraction processing technology is easily-controlled, simple and lower cost, and the final extraction concentration of total anthraquinone is up to 4.3231 mg/L under the processing technology above.
优化酶解提取总蒽醌的工艺。
以酶解提取大黄（掌叶大黄）中的总蒽醌浓度为实验评价指标，在单因素方差分析的基础上，分别考察酶用量、酶解液温度、酶解时间和pH值对总蒽醌提取率的影响。此外，基于响应面法对其提取工艺进行优化。
当酶用量高达0.12 g时，总蒽醌的得率最高（3.1730±0.1023 mg/L）；此外，对于酶解时间，总蒽醌得率随酶解时间的增加呈上升趋势，当酶解时间为48 h时，总蒽醌的最高浓度可达2.1410±0.1252 mg/L；而且，当pH值和酶解液温度分别达到4.8和50℃时，总蒽醌的最高含量分别可达2.2777±0.2133 mg/L和4.1360±0.3877 mg/L。最后，通过响应面法得到的最适酶解提取工艺如下：pH值为4.59，酶用量为0.1 g，酶解液温度为45.86℃。
该提取工艺条件易于控制，操作简单，成本较低，在上述工艺条件下总蒽醌的最终提取浓度可达4.3231 mg/L。
