Yang Sai-Fei, Sun Yin-Shi, Wang Jian-Hua
Zhong Yao Cai. 2014 May;37(5):871-5.
Ionic liquid based microwave-assisted extraction (ILMAE) was developed for the effective extraction of five anthraquinones namely aloeemodin, rhein, emodin, chrysophanol and physcion from Polygonum cuspidatum.
Experimental factors and levels were firstly selected by one-factor test. According to the central composite experimental design principle, the response surface methodology with three factors and five levels was adopted for comprehensive assessment by total extraction yield of five anthraquinones as a target.
The optimized parameters of MAE were: 0.6 mol/L [C8 MIM] Br, solvent to solid ratio of 40:1, power of 200 W, temperature of 31 degrees C and extraction time of 8 min. Under the optimal conditions, this approach gained total extraction yield of five anthraquinones 11.69 mg/g, while the predicted total extraction yield was 11.74 mg/g.
The research indicates ILMAE is an efficient, rapid, environment friendly and simple sample preparation technique to five anthraquinones from Polygonum cuspidatum.
开发基于离子液体的微波辅助萃取（ILMAE）方法，用于从虎杖中有效提取芦荟大黄素、大黄酸、大黄素、 Chrysophanol 和大黄酚这五种蒽醌类化合物。
首先通过单因素试验选择实验因素和水平。根据中心复合实验设计原理，采用三因素五水平的响应面法，以五种蒽醌类化合物的总提取率为指标进行综合评价。
微波辅助萃取的优化参数为：0.6 mol/L [C8 MIM] Br，料液比40:1，功率200 W，温度31℃，萃取时间8 min。在最佳条件下，该方法得到五种蒽醌类化合物的总提取率为11.69 mg/g，而预测的总提取率为11.74 mg/g。
研究表明ILMAE是一种高效、快速、环保且简单的从虎杖中提取五种蒽醌类化合物的样品制备技术。
