Liang Yu-Yong, Yin Shuang-Shuang, Zuo Bei-Mei, Gao Wen-Yuan
Tongren Polytechnic College, Tongren 554300, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(24):3803-7.
To systematically optimize the cultivation conditions of adventitious roots of Pseudostellaria heterophylla.
Tissue cultivation technology and ultraviolet spectrophotometry were adopted to observe the effect of inoculum volume, sucrose concentration, inorganic salt concentration, number of cultivation days, gradual scale-up cultivation and bubble different angles of bioreactor on the growth of adventitious roots of P. heterophylla, and determine the content of constituents such as saponin, polysaccharide and amino acid.
The propagation multiple of adventitious roots reached the maximum when the inoculum was 6 g in a 1 L culture shake flask. With the increase in sucrose concentration, the dry weight propagation multiples of adventitious roots followed an up and down trend. The inorganic salt concentration in a cultivation dish had a greater effect on the growth of adventitious roots, particularly 3/4 MS was the most favorable for the growth of adventitious roots. The growth curve of P. heterophylla was "S", with the biomass reaching the maximum at the 28th day.
The inoculum volume, sucrose concentration, inorganic salt concentration, gradual scale-up cultivation and angles of bubble bioreactor had a significant effect on the growth of adventitious roots of P. heterophylla. The contents of saponin and amino acid in adventitious roots were higher than that in cultivated P. heterophylla, whereas the polysaccharide content were lower than that in cultivated P. heterophylla.
系统优化太子参不定根的培养条件。
采用组织培养技术和紫外分光光度法，观察接种量、蔗糖浓度、无机盐浓度、培养天数、逐级放大培养及生物反应器气泡不同角度对太子参不定根生长的影响，并测定皂苷、多糖和氨基酸等成分的含量。
在1 L培养摇瓶中接种量为6 g时，不定根的增殖倍数达到最大。随着蔗糖浓度的增加，不定根的干重增殖倍数呈先上升后下降趋势。培养皿中的无机盐浓度对不定根生长影响较大，尤其3/4 MS最有利于不定根生长。太子参的生长曲线呈“S”形，在第28天生物量达到最大。
接种量、蔗糖浓度、无机盐浓度、逐级放大培养及生物反应器气泡角度对太子参不定根生长有显著影响。不定根中皂苷和氨基酸含量高于栽培太子参，而多糖含量低于栽培太子参。
