Department of Diagnosis and Surgery, Division of Periodontology, School of Dentistry, UNESP-Univ. Estadual Paulista, São Paulo, Brazil.
Wound Repair Regen. 2013 May-Jun;21(3):456-63. doi: 10.1111/wrr.12041. Epub 2013 Apr 29.
The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C--Control (nontreated), A--Artin M gel, and V--Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers.
凝集素 Artin M 已被证明可加速伤口愈合过程。本研究旨在评估 Artin M 对大鼠腭黏膜伤口愈合的影响，并研究 Artin M 对大鼠牙龈成纤维细胞转化生长因子 β（TGF-β）和血管内皮生长因子（VEGF）分泌的影响。在 72 只大鼠的腭黏膜上创建一个手术伤口，根据治疗方法将其分为三组：C-对照（未治疗）、A-Artin M 凝胶和 V-载体。每组 8 只动物在手术后 3、5 和 7 天处死进行组织学、免疫组织化学和细胞因子、生长因子水平的测定。用 2.5μg/mL 的 Artin M 孵育牙龈成纤维细胞 24、48 和 72 小时。通过酶联免疫吸附试验测定 VEGF 和 TGF-β的表达。组织学上，在第 7 天，Artin M 组表现出更早的上皮再形成、较轻的炎症浸润和增加的胶原纤维形成，导致颗粒组织更快成熟，与其他组相比（p<0.05）。Artin M 在体内诱导细胞增殖，并在两项实验中促进 TGF-β和 VEGF 的表达增加（p<0.05）。Artin M 可有效治疗大鼠口腔黏膜伤口，与 TGF-β和 VEGF 释放增加、细胞增殖、上皮再形成以及纤维胶原沉积和排列有关。
