Blood Center of Zhejiang Province, Hangzhou, Zhejiang, China; Key laboratory of blood safety research, Ministry of Health, Hangzhou, Zhejiang, China.
Int J Immunogenet. 2014 Feb;41(1):13-9. doi: 10.1111/iji.12064. Epub 2013 Jun 4.
Umbilical cord blood (UCB) is a widely accepted source of progenitor cells, and now, many cord blood banks were established. Here, we analysed the HLA-A, HLA-B and HLA-DRB1 allele and haplotype frequencies, HLA matching possibilities for searching potential donors and outcome of UCB transplantations in Zhejiang cord blood bank of China. A total of 6384 UCB units were characterized for 17 HLA-A, 30 HLA-B and 13 HLA-DRB1 alleles at the first field resolution level. Additionally, B14, B15 and B40 were typed to the second field level. A total of 1372 distinct A-B-DRB1 haplotypes were identified. The frequencies of 7 haplotypes were more than 1%, and 439 haplotypes were <0.01%. A02-B46-DRB109, A33-B58-DRB103 and A30-B13-DRB107 were the most common haplotypes, with frequencies of 4.4%, 3.3%, and 2.9%, respectively. Linkage disequilibrium(LD) analysis showed that there were 83 A-B, 106 B-DRB1, 54 A-DRB1 haplotypes with positive LD, in which 51 A-B, 60 B-DRB1, 32 A-DRB1 haplotypes exhibited a significant LD (P < 0.05). In 682 search requests, 12.9%, 40.0% and 42.7% of patients were found to have 6 of 6, 5 of 6 and 4 of 6 HLA-A, HLA-B and HLA-DRB1 matching donors, respectively. A total of 30 UCB units were transplanted to 24 patients (3 patients not evaluated due to early death); 14 of 21 patients (66.7%) engrafted. This study reveals the HLA distribution and its transplantation application in the cord blood bank of Zhejiang province. These data can help to select potential UCB donors for transplantation and used to assess the scale of new cord blood banking endeavours.
脐带血（UCB）是一种广泛接受的祖细胞来源，现在已经建立了许多脐带血库。在这里，我们分析了中国浙江脐带血库的 HLA-A、HLA-B 和 HLA-DRB1 等位基因和单倍型频率、寻找潜在供体的 HLA 匹配可能性以及 UCB 移植的结果。在第一字段分辨率水平上，对 6384 个 UCB 单位进行了 17 个 HLA-A、30 个 HLA-B 和 13 个 HLA-DRB1 等位基因的特征分析。此外，B14、B15 和 B40 被分型到第二字段水平。共鉴定出 1372 个独特的 A-B-DRB1 单倍型。7 种单倍型的频率超过 1%，439 种单倍型的频率<0.01%。A02-B46-DRB109、A33-B58-DRB103 和 A30-B13-DRB107 是最常见的单倍型，频率分别为 4.4%、3.3%和 2.9%。连锁不平衡（LD）分析显示，有 83 个 A-B、106 个 B-DRB1、54 个 A-DRB1 单倍型存在正 LD，其中 51 个 A-B、60 个 B-DRB1、32 个 A-DRB1 单倍型存在显著 LD（P<0.05）。在 682 个搜索请求中，分别有 12.9%、40.0%和 42.7%的患者发现有 6/6、5/6 和 4/6 HLA-A、HLA-B 和 HLA-DRB1 匹配的供体。共移植了 30 个 UCB 单位给 24 名患者（由于早期死亡，3 名患者未评估）；21 名患者中的 14 名（66.7%）植入。本研究揭示了浙江省脐带血库的 HLA 分布及其在移植中的应用。这些数据有助于选择潜在的 UCB 供体进行移植，并用于评估新的脐带血库建设工作的规模。
