Separation Engineering of Chinese Traditional Medicine Compound, Nanjing University of Chinese Medicine, Nanjing 210029, China.
Phytomedicine. 2013 Jul 15;20(10):767-74. doi: 10.1016/j.phymed.2012.11.012. Epub 2013 Apr 28.
Our previous studies showed that after oral administration of an Huang-Lian-Jie-Du-Tang (HLJDT) decoction, there is a higher concentration of the pure components, berberine, baicalin and gardenoside in the plasma of Middle cerebral artery occlusion (MCAO) rats than in sham-operated rats, The aim of the present study was to determine whether these components could be reliably measured in MCAO rat tissues. First, the plasma concentration-time profiles of berberine, palmatine, baicalin, baicalein and gardenoside were characterised in MCAO rats after oral administration of the aqueous extract of HLJDT. Subsequently, liver, lung and kidney tissues were obtained from sudden death MCAO rats in the absorption phase (0.25 h), the distribution phase (1.0 h) and the elimination phase (8.0 h) after administration of the HLJDT aqueous extract. An HPLC method was developed and validated for the determination of the distribution characteristics of berberine, palmatine, baicalin, baicalein and gardenoside simultaneously from the above-mentioned rat tissues. The results indicated that berberine, palmatine, baicalin and baicalein distributed rapidly and accumulated at high levels in the lung, while gardenoside distributed widely in the lung and the kidney. To the best of our knowledge, this is the first report to describe the distribution of the active ingredients derived from HLJDT in MCAO rat tissues. The tissue distribution results provide a biopharmaceutical basis for the design of the clinic application of HLJDT in cerebrovascular disease.
我们之前的研究表明，口服黄连解毒汤（HLJDT）后，在大脑中动脉闭塞（MCAO）大鼠的血浆中，黄连、黄芩苷和栀子苷等纯成分的浓度高于假手术大鼠。本研究旨在确定这些成分是否可以在 MCAO 大鼠组织中可靠地测量。首先，在口服 HLJDT 水提物后，研究了 MCAO 大鼠体内的黄连素、巴马汀、黄芩苷、黄芩素和栀子苷的血浆浓度-时间曲线。随后，在口服 HLJDT 水提物后吸收相（0.25 h）、分布相（1.0 h）和消除相（8.0 h），从突然死亡的 MCAO 大鼠中获得肝脏、肺和肾脏组织。建立并验证了一种 HPLC 方法，用于同时测定上述大鼠组织中黄连素、巴马汀、黄芩苷、黄芩素和栀子苷的分布特征。结果表明，黄连素、巴马汀、黄芩苷和黄芩素在肺中快速分布并积累高浓度，而栀子苷在肺和肾中广泛分布。据我们所知，这是首次描述 HLJDT 活性成分在 MCAO 大鼠组织中的分布情况。组织分布结果为 HLJDT 在脑血管疾病临床应用的设计提供了生物制药基础。
