Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany.
Meat Sci. 2013 Aug;94(4):474-9. doi: 10.1016/j.meatsci.2013.04.015. Epub 2013 Apr 13.
The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A>G polymorphism significantly associated with pH 24(H), meat percentage and muscle area in the F₂ Duroc × Pietrain (DuPi, n=313) and with pH 24(L), fat area and backfat thickness in the Pietrain (Pi, n=110) population. HNF1A mRNA and protein expressions were higher (p<0.05) in animals with the low post-mortem muscle pH 24(L). The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p>0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect.
本研究旨在探讨 HNF1A 基因作为候选基因与猪肉质和胴体性状的相关性和表达。统计分析表明，g.8260A>G 多态性与 F₂杜洛克×皮特兰猪（DuPi，n=313）的 24 小时 pH 值 24（H）、肌肉百分比和肌肉面积以及皮特兰猪（Pi，n=110）的 24 小时 pH 值 24（L）、脂肪面积和背膘厚度显著相关。低宰后肌肉 pH 值 24（L）的动物中 HNF1A mRNA 和蛋白表达水平较高（p<0.05）。启动子甲基化分析表明，在肌肉 pH 值不同的动物中，甲基化不参与 HNF1A 表达调控（p>0.05）。总之，猪 HNF1A 基因的多态性可作为改善肉质和胴体性状的候选标记，需考虑特定品种的影响。
