Zhang Rui, Große-Brinkhaus Christine, Heidt Hanna, Uddin Muhammad Jasim, Cinar Mehmet Ulas, Tesfaye Dawit, Tholen Ernst, Looft Christian, Schellander Karl, Neuhoff Christiane
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany.
Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany; Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Meat Sci. 2015 Sep;107:26-32. doi: 10.1016/j.meatsci.2015.04.007. Epub 2015 Apr 22.
The aim of the study was to investigate single nucleotide polymorphisms (SNPs) and expression of SOX-6 to support its candidacy for growth, carcass, and meat quality traits in pigs. The first SNP, rs81358375, was associated with pH 45 min post mortem in loin (pH1L), the thickness of backfat and side fat, and carcass length in Pietrain (Pi) population, and related with backfat thickness and daily gain in Duroc × Pietrain F2 (DuPi) population. The other SNP, rs321666676, was associated with meat colour in Pi population. In DuPi population, the protein, not mRNA, level of SOX-6 in high pH1L pigs was significantly less abundant compared with low pH1L pigs, where microRNAs targeting SOX-6 were also differently regulated. This paper shows that SOX-6 could be a potential candidate gene for porcine growth, carcass, and meat quality traits based on genetic association and gene expression.
本研究的目的是调查单核苷酸多态性（SNP）以及SOX-6的表达情况，以支持其作为猪生长、胴体和肉质性状候选基因的可能性。第一个SNP，即rs81358375，与皮特兰（Pi）群体中腰大肌宰后45分钟的pH值（pH1L）、背膘和侧膘厚度以及胴体长度相关，并且与杜洛克×皮特兰F2（DuPi）群体中的背膘厚度和日增重相关。另一个SNP，即rs321666676，与Pi群体中的肉色相关。在DuPi群体中，高pH1L猪的SOX-6蛋白水平（而非mRNA水平）明显低于低pH1L猪，而且靶向SOX-6的微小RNA的调控方式也有所不同。基于遗传关联和基因表达，本文表明SOX-6可能是猪生长、胴体和肉质性状的一个潜在候选基因。
