Laboratory of Veterinary Pharmacokinetics & Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
Genomics. 2013 Jul;102(1):47-56. doi: 10.1016/j.ygeno.2013.04.011. Epub 2013 Apr 26.
In the present study, a computational comparative and subtractive genomic/proteomic analysis aimed at the identification of putative therapeutic target and vaccine candidate proteins from Kyoto Encyclopedia of Genes and Genomes (KEGG) annotated metabolic pathways of Mycoplasma hyopneumoniae was performed for drug design and vaccine production pipelines against M.hyopneumoniae. The employed comparative genomic and metabolic pathway analysis with a predefined computational systemic workflow extracted a total of 41 annotated metabolic pathways from KEGG among which five were unique to M. hyopneumoniae. A total of 234 proteins were identified to be involved in these metabolic pathways. Although 125 non homologous and predicted essential proteins were found from the total that could serve as potential drug targets and vaccine candidates, additional prioritizing parameters characterize 21 proteins as vaccine candidate while druggability of each of the identified proteins evaluated by the DrugBank database prioritized 42 proteins suitable for drug targets.
在本研究中，我们进行了一项计算比较和消减基因组/蛋白质组分析，旨在从京都基因与基因组百科全书（KEGG）注释的猪肺炎支原体代谢途径中鉴定潜在的治疗靶标和疫苗候选蛋白，以用于药物设计和疫苗生产针对 M. hyopneumoniae 的管道。所采用的比较基因组和代谢途径分析，以及预定义的计算系统工作流程，从 KEGG 中总共提取了 41 条注释代谢途径，其中 5 条是 M. hyopneumoniae 特有的。总共鉴定出 234 种蛋白质参与这些代谢途径。尽管从总数中发现了 125 种非同源和预测必需的蛋白质，它们可以作为潜在的药物靶点和疫苗候选物，但额外的优先级参数将 21 种蛋白质特征化为疫苗候选物，而通过 DrugBank 数据库评估的每种鉴定蛋白质的可药性则将 42 种蛋白质优先用于药物靶点。
