Budde U, Schneppenheim R, Plendl H, Dent J, Ruggeri Z M, Zimmerman T S
Blood Transfusion Service AK Harburg, Hamburg, FRG.
Thromb Haemost. 1990 Apr 12;63(2):312-5.
Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, IIE, and IIF. Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf:Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf:Ag detectable after 1:300 dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.
针对血管性血友病因子（vWf）多聚体可视化的两种方法，在正常vWf以及vWd变体IIA、IIB、IIC、IID、IIE和IIF的敏感性和检测方面进行了比较。将vWf多聚体电转移至硝酸纤维素膜后进行放射自显影和发光成像，结果显示二者敏感性相当，正常血浆稀释1:500后仍可检测到vWf:Ag。最不敏感的方法是琼脂糖凝胶中的发光成像，正常血浆稀释1:300后才能检测到vWf:Ag。vWd变体患者血浆的条带模式没有差异。
