pri-miR-34b/c 的启动子多态性与肝细胞癌相关。

Promoter polymorphisms of pri-miR-34b/c are associated with hepatocellular carcinoma.

机构信息

Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.

出版信息

Gene. 2013 Jul 25;524(2):156-60. doi: 10.1016/j.gene.2013.04.042. Epub 2013 Apr 28.

DOI:10.1016/j.gene.2013.04.042

PMID:23632240

Abstract

BACKGROUND

Numerous studies have focused on the association between miR-34 family members, which are direct p53 targets, and carcinogenesis of many cancers, including hepatocellular carcinoma (HCC). This study aimed to assess whether polymorphisms in the single-nucleotide polymorphism miR-34b/c T>C (rs4938723) and TP53 Arg72Pro (rs1042522) increase the risk of HCC and influence outcome in patients with HCC.

PATIENTS AND METHODS

We enrolled 157 HCC patients and 201 cancer-free control subjects from the Korean population. MicroRNA polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

RESULTS

We found that the miR-34b/c TC+CC frequency was significantly higher in HCC patients than in controls (adjusted odds ratio [AOR]: 1.580; 95% confidence interval [CI]: 1.029-2.426). The miR-34b/c CC-TP53 Arg/Arg combination significantly increased the risk of HCC (AOR: 13.644; 95% CI: 1.451-128.301). The SNPs miR-34b/c T>C and TP53 Arg72Pro(G>C) had no influence on survival of HCC patients.

CONCLUSIONS

Our findings suggest that loss of the T allele in miR-34b/c T>C, and the miR-34b/c CC-TP53 Arg/Arg combination increases the risk of HCC in the Korean population.

摘要

背景

许多研究集中在 miR-34 家族成员与多种癌症（包括肝细胞癌 [HCC]）的致癌作用之间的关联上，miR-34 家族成员是 p53 的直接靶标。本研究旨在评估单核苷酸多态性 miR-34b/c T>C（rs4938723）和 TP53 Arg72Pro（rs1042522）中的多态性是否增加 HCC 的风险，并影响 HCC 患者的预后。

患者与方法

我们从韩国人群中招募了 157 名 HCC 患者和 201 名无癌症对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测 miRNA 多态性。

结果

我们发现 HCC 患者 miR-34b/c TC+CC 频率明显高于对照组（调整后的优势比 [AOR]：1.580；95%置信区间 [CI]：1.029-2.426）。miR-34b/c CC-TP53 Arg/Arg 组合显著增加 HCC 的发病风险（AOR：13.644；95%CI：1.451-128.301）。miR-34b/c T>C 和 TP53 Arg72Pro（G>C）的 SNPs 对 HCC 患者的生存无影响。