Department and Clinic of Paediatric Surgery and Urology, Wroclaw Medical University, ul. M.Sklodowskiej-Curie 50/52, 50-369, Wrocław, Poland.
Int Urol Nephrol. 2013 Jun;45(3):607-12. doi: 10.1007/s11255-013-0454-7. Epub 2013 Apr 30.
The authors analysed the distribution of c-kit-positive interstitial cells of Cajal (ICCs) in obstructed ureteropelvic junction (UPJ) and its age-related changes.
Twenty specimens were obtained from children with intrinsic ureteropelvic junction obstruction (UPJO), at the average age of 8.1 years (8 months-16.8 years), fixed in formalin and embedded in paraffin. Five control samples were taken from children at the average age of 2.3 years (2.4 months-7.4 years). All specimens were analysed by the immunohistochemistry test with light microscopy with respect to c-kit expression. The distribution of c-kit-positive ICCs in the two groups was compared and the correlation between the distribution of c-kit-positive ICCs and the patients' age in UPJO cases was analysed. The results were examined by Yates' χ(2) test, Mann-Whitney U test, and t test for Pearson's correlation coefficient. A P value < 0.05 was considered as statistically significant.
No statistically significant differences were found in the distribution of c-kit-positive ICCs between UPJO and the control group. No correlation was established between the age of patients with UPJO and the distribution of c-kit-positive ICCs.
No distributional difference found in obstructed and unobstructed UPJ seems to indicate that UPJO is not associated with anomalous distribution of c-kit-positive ICCs. Age-related changes in the expression of c-kit-positive ICCs are equally distributed in obstructed UPJ.
作者分析了在梗阻性肾盂输尿管连接部（UPJ）中 c-kit 阳性的间质 Cajal 细胞（ICC）的分布及其与年龄相关的变化。
从患有内在性肾盂输尿管连接部梗阻（UPJO）的儿童中获得 20 个标本，平均年龄为 8.1 岁（8 个月至 16.8 岁），用福尔马林固定并石蜡包埋。从平均年龄为 2.3 岁（2.4 个月至 7.4 岁）的儿童中获得 5 个对照样本。所有标本均通过免疫组织化学测试，用显微镜观察 c-kit 表达。比较两组 c-kit 阳性 ICC 的分布，并分析 UPJO 病例中 c-kit 阳性 ICC 的分布与患者年龄之间的相关性。使用 Yates χ(2)检验、Mann-Whitney U 检验和 Pearson 相关系数的 t 检验来检验结果。P 值<0.05 被认为具有统计学意义。
在 UPJO 和对照组之间，c-kit 阳性 ICC 的分布没有统计学差异。在 UPJO 患者的年龄与 c-kit 阳性 ICC 的分布之间没有建立相关性。
在梗阻和非梗阻 UPJ 之间没有发现分布差异，这似乎表明 UPJO 与 c-kit 阳性 ICC 的异常分布无关。在梗阻性 UPJ 中，c-kit 阳性 ICC 表达的年龄相关变化均匀分布。
