Watt P J, Robinson B S, Pringle C R, Tyrrell D A
Department of Microbiology, Southampton University Medical School, Southampton General Hospital, UK.
Vaccine. 1990 Jun;8(3):231-6. doi: 10.1016/0264-410x(90)90051-m.
The virulence and immunogenicity of a wild-type respiratory syncytial (RS) virus together with four temperature sensitive (ts) mutants derived from this isolate were tested by intranasal inoculation into adult volunteers. Resistance to challenge correlated with neutralizing antibody titres in nasal secretions and to a lesser extent in serum. All ts mutants were reduced in virulence. Mutant ts1B caused mild or asymptomatic infections yet induced antibody responses comparable with wild-type RS virus. Ts1B might be developed further to produce a live-virus vaccine.
通过对成年志愿者进行鼻内接种，测试了野生型呼吸道合胞（RS）病毒以及从该分离株衍生的四个温度敏感（ts）突变体的毒力和免疫原性。对攻击的抵抗力与鼻分泌物中的中和抗体滴度相关，在血清中的相关性较小。所有ts突变体的毒力均降低。突变体ts1B引起轻度或无症状感染，但诱导的抗体反应与野生型RS病毒相当。ts1B可能会进一步开发以生产活病毒疫苗。
