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用间接血凝试验诊断牛的牛血吸虫感染

Diagnosis of Schistosoma bovis infection in cattle by an indirect haemagglutination test.

作者信息

Preston J M, Duffus W P

出版信息

J Helminthol. 1975 Mar;49(1):9-17.

PMID:236339
Abstract

An indirect haemagglutination test has been developed for the detection of Schistosoma bovis antibody. Fresh, formalin and glutaraldehyde fixed sheep erythrocytes as well as fresh and formalin fixed human erythrocytes were tested. Tanned glutaraldehyde fixed sheep erythrocytes sensitized with an adult worm extract were the most sensitive and remained stable for at least 8 weeks at 4 degrees C. With such cells 98% of a group of S. bovis infected cattle were positive while negative results were obtained from cattle infected with other helminths.

摘要

已开发出一种间接血凝试验用于检测牛血吸虫抗体。对新鲜的、经福尔马林和戊二醛固定的绵羊红细胞以及新鲜的和经福尔马林固定的人红细胞进行了测试。用成虫提取物致敏的经鞣酸处理的戊二醛固定绵羊红细胞最为敏感,在4℃下至少可稳定8周。用这种细胞检测时,一组感染牛血吸虫的牛中有98%呈阳性,而感染其他蠕虫的牛则呈阴性。

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