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间接血凝试验,该试验使用经提取物抗原致敏的戊二醛固定羊红细胞来检测巴氏杆菌抗体。

Indirect hemagglutination test that uses glutaraldehyde-fixed sheep erythrocytes sensitized with extract antigens for detection of Pasteurella antibody.

作者信息

Sawada T, Rimler R B, Rhoades K R

出版信息

J Clin Microbiol. 1982 May;15(5):752-6. doi: 10.1128/jcm.15.5.752-756.1982.

Abstract

Glutaraldehyde-fixed sheep erythrocytes (GA-SRBC) were used in the indirect hemagglutination test for the detection of Pasteurella antibody. GA-SRBC were stable for at least 6 months. Heat extract or potassium thiocyanate extract antigens of Pasteurella strains could be adsorbed onto GA-SRBC or tanned GA-SRBC, respectively. The indirect hemagglutination test reaction was capsular group specific with heat extract antigen-sensitized GA-SRBC but not potassium thiocyanate extract antigen-sensitized tanned GA-SRBC.

摘要

戊二醛固定的绵羊红细胞(GA-SRBC)用于间接血凝试验检测巴氏杆菌抗体。GA-SRBC至少稳定6个月。巴氏杆菌菌株的热提取物或硫氰酸钾提取物抗原可分别吸附到GA-SRBC或鞣酸处理的GA-SRBC上。间接血凝试验反应对于热提取物抗原致敏的GA-SRBC具有荚膜群特异性,但对于硫氰酸钾提取物抗原致敏的鞣酸处理的GA-SRBC则不具有荚膜群特异性。

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