Swolin B, Rödjer S, Westin J
Department of Clinical Chemistry, Sahlgrenska Hospital, Gothenburg, Sweden.
Am J Hematol. 1990 Jul;34(3):175-80. doi: 10.1002/ajh.2830340304.
Thirty-eight consecutive patients with a FAB-classified primary myelodysplastic syndrome (MDS) were investigated for in vitro growth of colony-forming units for granulocyte-macrophage precursors (CFU-GM) and cytogenetic analysis of bone marrow cells. Abnormal CFU-GM growth was found in 30 patients (79%), and clonal chromosome abnormalities were found in 13 patients (34%). The eight patients who showed normal CFU-GM growth were either cytogenetically normal (n = 5), or had a 5q-deletion (n = 3) as single or dominating karyotypic abnormality. Among the 30 patients with reduced or no colony growth, ten patients had a clonal chromosome abnormality. Leukemia developed in eight patients. None of them grew any CFU-GM colonies, and three of them were cytogenetically abnormal at the time of diagnosis of MDS. Analysis of the bone marrow in vitro growth for CFU-GM and the karyotype in patients with MDS emphasizes the close relationship between these disorders and manifest acute leukemia. Subgroups of MDS may be defined by a cytogenetic classification (e.g., the 5q-syndrome), and the CFU-GM growth pattern can be of value for predicting leukemic transformation.
对38例经FAB分类的原发性骨髓增生异常综合征（MDS）患者进行了粒细胞-巨噬细胞前体集落形成单位（CFU-GM）的体外生长研究及骨髓细胞的细胞遗传学分析。30例患者（79%）发现CFU-GM生长异常，13例患者（34%）发现克隆性染色体异常。CFU-GM生长正常的8例患者，要么细胞遗传学正常（n = 5），要么有5q缺失（n = 3）作为单一或主要的核型异常。在30例集落生长减少或无生长的患者中，10例有克隆性染色体异常。8例患者发生了白血病。他们均未生长出任何CFU-GM集落，其中3例在MDS诊断时细胞遗传学异常。对MDS患者CFU-GM的骨髓体外生长及核型分析强调了这些疾病与明显急性白血病之间的密切关系。MDS的亚组可通过细胞遗传学分类来定义（如5q综合征），CFU-GM生长模式对预测白血病转化可能有价值。
