金黄色葡萄球菌甲醛灭活肠毒素A和C1的免疫原性

Immunogenicity of formaldehyde-inactivated enterotoxins A and C1 of Staphylococcus aureus.

作者信息

Warren J R, Spero L, Metzger J F, McGann V G

出版信息

J Infect Dis. 1975 May;131(5):535-42. doi: 10.1093/infdis/131.5.535.

DOI:10.1093/infdis/131.5.535

Abstract

Quantitative precipitation of antisera specific for native enterotoxin revealed that 70% and 60%, respectively, of the antigenic determinants of enterotoxins A and C1 of Staphylococcus aureus were inactivated by formaldehyde at pH 5.0 or 7.5 contained large polymers (excluded by Sepharose 2B) and induced strong humoral immune responses in rhesus monkeys. Enterotoxin A inactivated at pH 5.0 or 7.5 was composed mostly of small polymers (excluded by Sephadex G-100 but included by Sepharose 2B); it provoked a poor immune response in monkeys (about equivalent to the response obtained with weakly reactive toxin inactivated at alkaline pH). It was concluded that potent enterotoxoids were formed by extensive cross-linking of enterotoxin C1 into large polymers in acidic or neutral formaldehyde solution.

摘要

针对天然肠毒素的抗血清定量沉淀显示，金黄色葡萄球菌肠毒素A和C1的抗原决定簇分别有70%和60%在pH 5.0或7.5时被甲醛灭活，含有大聚合物（被琼脂糖2B排除），并在恒河猴中诱导强烈的体液免疫反应。在pH 5.0或7.5时灭活的肠毒素A主要由小聚合物组成（被葡聚糖G - 100排除但被琼脂糖2B包含）；它在猴子中引发的免疫反应较差（约相当于用在碱性pH下灭活的弱反应性毒素获得的反应）。得出的结论是，通过在酸性或中性甲醛溶液中将肠毒素C1广泛交联成大聚合物形成了有效的类毒素。