Forsgren A, Forsum U, Hallander H O
Appl Microbiol. 1972 Mar;23(3):559-64. doi: 10.1128/am.23.3.559-564.1972.
Enterotoxin B-producing and -nonproducing Staphylococcus aureus strains showed cell fluorescence when tested with fluoresceinisothiocyanate-labeled rabbit anti-enterotoxin B globulin, probably as a result of a protein A-immunoglobulin G (Ig G) interaction. No cell-bound enterotoxin B could be detected by immunofluorescence using F(ab(1))(2)-fragments of anti-enterotoxin B globulin. However, soluble enterotoxin B could be estimated by immunofluorescence. Approximately 1,000-fold more enterotoxin B was detected by immunodiffusion as an extracellular product in the media than could be detected in the cell fraction. The results show that intact Ig G is not suitable for the detection of antigens other than protein A on the cell surface of S. aureus in conventional immunofluorescence. For such purposes, the use of F(ab(1))(2)-fragments of Ig G is recommended.
用异硫氰酸荧光素标记的兔抗肠毒素B球蛋白检测时，产肠毒素B和不产肠毒素B的金黄色葡萄球菌菌株均呈现细胞荧光，这可能是蛋白A -免疫球蛋白G（IgG）相互作用的结果。使用抗肠毒素B球蛋白的F(ab(1))(2)片段进行免疫荧光检测时，未检测到细胞结合的肠毒素B。然而，可溶性肠毒素B可通过免疫荧光进行估算。通过免疫扩散检测到培养基中作为细胞外产物的肠毒素B比细胞组分中检测到的大约多1000倍。结果表明，在传统免疫荧光中，完整的IgG不适用于检测金黄色葡萄球菌细胞表面除蛋白A以外的抗原。为此，建议使用IgG的F(ab(1))(2)片段。
