通过毛细管电泳对DNA测序反应进行高速分离。

High-speed separations of DNA sequencing reactions by capillary electrophoresis.

作者信息

Drossman H, Luckey J A, Kostichka A J, D'Cunha J, Smith L M

机构信息

Department of Chemistry, University of Wisconsin, Madison 53706.

出版信息

Anal Chem. 1990 May 1;62(9):900-3. doi: 10.1021/ac00208a003.

DOI:10.1021/ac00208a003

PMID:2363514

Abstract

Fluorescently labeled DNA fragments generated in enzymatic sequencing reactions are rapidly separated by capillary gel electrophoresis and detected at attomole levels within the gel-filled capillary. The application of this technology to automated DNA sequence analysis may permit the development of a second generation automated sequencer capable of efficient and cost-effective sequence analysis on the genomic scale.

摘要

在酶促测序反应中产生的荧光标记DNA片段通过毛细管凝胶电泳快速分离，并在充满凝胶的毛细管内以阿托摩尔水平进行检测。将该技术应用于自动化DNA序列分析可能会促使开发出第二代自动化测序仪，能够在基因组规模上进行高效且经济高效的序列分析。