Durney Brandon C, Crihfield Cassandra L, Holland Lisa A
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.
Anal Bioanal Chem. 2015 Sep;407(23):6923-38. doi: 10.1007/s00216-015-8703-5. Epub 2015 May 3.
This review of capillary electrophoresis methods for DNA analyses covers critical advances from 2009 to 2014, referencing 184 citations. Separation mechanisms based on free-zone capillary electrophoresis, Ogston sieving, and reptation are described. Two prevalent gel matrices for gel-facilitated sieving, which are linear polyacrylamide and polydimethylacrylamide, are compared in terms of performance, cost, viscosity, and passivation of electroosmotic flow. The role of capillary electrophoresis in the discovery, design, and characterization of DNA aptamers for molecular recognition is discussed. Expanding and emerging techniques in the field are also highlighted.
本对用于DNA分析的毛细管电泳方法的综述涵盖了2009年至2014年的关键进展，参考文献184篇。描述了基于自由区毛细管电泳、奥格斯顿筛分和蠕动的分离机制。比较了用于凝胶辅助筛分的两种常见凝胶基质，即线性聚丙烯酰胺和聚二甲基丙烯酰胺，在性能、成本、粘度和电渗流钝化方面的情况。讨论了毛细管电泳在用于分子识别的DNA适体的发现、设计和表征中的作用。还强调了该领域不断扩展和新兴的技术。
