State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing, 210098, China.
Curr Microbiol. 2013 Sep;67(3):327-32. doi: 10.1007/s00284-013-0369-7. Epub 2013 May 1.
In order to characterize the vertical variation of abundance and community composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in sediments of a eutrophic lake, Lake Taihu, molecular techniques including real-time PCR, clone library, and sequencing were carried out in this study. Abundances of archaeal amoA gene (ranged from 2.34 × 10(6) to 4.43 × 10(7) copies g dry sediment) were higher than those of bacterial amoA gene (ranged from 5.02 × 10(4) to 6.91 × 10(6) copies g dry sediment) for all samples and both of them exhibited negative correlations with the increased depths. Diversities of archaeal and bacterial amoA gene increased with the elevated depths. There were no significant variations of AOB community structures derived from different sediment depths, whereas obvious differences were observed for the AOA community compositions. The information acquired in this study would be useful to elucidate the roles of AOA and AOB in the nitrogen cycling of freshwater ecosystems.
为了描述富营养化湖泊太湖沉积物中氨氧化古菌（AOA）和氨氧化细菌（AOB）的丰度和群落组成的垂直变化，本研究采用实时 PCR、克隆文库和测序等分子技术进行了研究。古菌 amoA 基因的丰度（范围为 2.34×10(6) 到 4.43×10(7) 拷贝 g 干沉积物)高于细菌 amoA 基因的丰度（范围为 5.02×10(4) 到 6.91×10(6) 拷贝 g 干沉积物)，所有样品均呈负相关，且随着深度的增加而降低。古菌和细菌 amoA 基因的多样性随深度的增加而增加。不同深度的 AOB 群落结构没有明显变化，而 AOA 群落组成则有明显差异。本研究获得的信息将有助于阐明 AOA 和 AOB 在淡水生态系统氮循环中的作用。
