Zeng Jin, Yang Liu-Yan, Liang Yi, Li Jia-Yun, Xiao Lin, Jiang Li-Juan, Zhao Da-Yong
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
Can J Microbiol. 2008 Dec;54(12):1053-63. doi: 10.1139/W08-098.
Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted with multivariate statistical analysis. Five major variables in sediment were examined in a principal component analysis, which indicates notable differences of physicochemical parameters among different sites of the lake. To assess changes in the genetic diversity of bacterial communities of different sampling sites, DGGE band patterns were analyzed by multidimensional scaling analysis, which indicated that sampling sites having similar environmental characteristics also have the similar microbial communities. Canonical correspondence analysis demonstrated that pH and redox potential had significant effects on the bacterial community composition in the sediments. Analysis of DNA sequences revealed that the dominant bacterial groups in Lake Xuanwu belonged to Proteobacteria, Actinobacteria, Verrucomicrobia, and Nitrospirae, which are commonly isolated from freshwater ecosystems.
对富营养化的玄武湖沉积物中的细菌群落结构以及几种环境因素对微生物群落分布的影响进行了研究。使用变性梯度凝胶电泳（DGGE）生成细菌群落图谱，并通过多变量统计分析对结果进行解释。在主成分分析中检查了沉积物中的五个主要变量，这表明该湖不同地点的理化参数存在显著差异。为了评估不同采样点细菌群落遗传多样性的变化，通过多维尺度分析对DGGE条带模式进行了分析，结果表明具有相似环境特征的采样点也具有相似的微生物群落。典范对应分析表明，pH值和氧化还原电位对沉积物中的细菌群落组成有显著影响。DNA序列分析表明，玄武湖中的优势细菌类群属于变形菌门、放线菌门、疣微菌门和硝化螺旋菌门，这些类群通常从淡水生态系统中分离得到。
