在三种不同人类细胞中鉴定与DNA连接酶I相关的多肽。

Identification of DNA ligase I related polypeptides in three different human cells.

作者信息

Prigent C, Aoufouchi S, Philippe M

机构信息

Laboratoire de Biologie et Génétique du Développement URA CNRS 256, Université de Rennes I, France.

出版信息

Biochem Biophys Res Commun. 1990 Jun 29;169(3):888-95. doi: 10.1016/0006-291x(90)91976-y.

DOI:10.1016/0006-291x(90)91976-y

PMID:2363731

Abstract

Partial purification of the DNA ligase from three human tissues (liver, thymus and lymphoblasts) revealed that each cell type contains several different polypeptides bearing a DNA ligase I activity. Their apparent molecular weights estimated after SDS PAGE, 130 kDa, 100 kDa and 80 kDa, are in agreement with previous reports. These polypeptides are related by proteolysis to a single higher molecular weight protein of 200 kDa which does not show DNA ligase activity but that could be a preprotein.

摘要

从三种人体组织（肝脏、胸腺和淋巴母细胞）中对DNA连接酶进行部分纯化后发现，每种细胞类型都含有几种具有DNA连接酶I活性的不同多肽。经SDS-PAGE后估计，它们的表观分子量分别为130 kDa、100 kDa和80 kDa，这与之前的报道一致。这些多肽通过蛋白水解与一种分子量更高的200 kDa单一蛋白质相关，该蛋白质不具有DNA连接酶活性，但可能是一种前体蛋白。