Jain Rakesh K, Munn Lance L, Fukumura Dai
Cold Spring Harb Protoc. 2013 May 1;2013(5):444-6. doi: 10.1101/pdb.prot074344.
Noninvasive techniques have been developed for the assessment of vascular parameters, including vascular permeability, in normal and diseased tissues of mice. In this protocol, mice are injected with an appropriate fluorescent tracer. The effective average vascular permeability (P) of a region of vessels is measured by single-photon microscopy. Additionally, the vascular permeability of individual vessel segments within ~600 µm of a tumor/window interface is measured by multiphoton laser-scanning microscopy (MPLSM).
已经开发出非侵入性技术来评估小鼠正常组织和患病组织中的血管参数，包括血管通透性。在本方案中，给小鼠注射适当的荧光示踪剂。通过单光子显微镜测量血管区域的有效平均血管通透性（P）。此外，通过多光子激光扫描显微镜（MPLSM）测量肿瘤/窗口界面约600 µm内单个血管段的血管通透性。
