Kishore Kundan, Rahman H, Kalita H, Pandey Brijesh, Monika N
ICAR Research Complex for NEH Region, Sikkim Centre, Tadong, Gangtok, 737102 Sikkim India.
Indian J Virol. 2010 Oct;21(2):140-3. doi: 10.1007/s13337-010-0019-7. Epub 2011 Jan 6.
The assessment of Citrus tristeza virus incidence in mandarin of Sikkim, involving sampling techniques, was estimated by DAS-ELISA. Mandarin orchards had high CTV incidence (46.32%), however, differential prevalence with regard to age of plant and location was observed. The CTV prevalence was relatively high in older orchards (51.01%) than that of younger ones (40.80%). Under all the plant age groups, south district had the highest CTV incidence (52.50%) and east district had the lowest (37.71%). The spatial distribution of CTV in plants indicates high concentration in twig followed by leaf tissue, however, stem had relatively less concentration. High aphid infestation was observed in all mandarin growing groves with the maximum in south district and minimum in east district. Taxoptera citricida was the predominating aphid species followed by T. aurantii, however, Aphis spp population was significantly less. Aphid infestation and CTV prevalence were positively and significantly correlated.
通过双抗夹心酶联免疫吸附测定法（DAS-ELISA）对锡金邦柑橘园中柑橘衰退病毒（CTV）的发病率进行了评估，评估涉及采样技术。柑橘园的CTV发病率很高（46.32%），不过，观察到发病率在植株年龄和种植地点方面存在差异。老果园的CTV发病率（51.01%）相对高于年轻果园（40.80%）。在所有植株年龄组中，南区的CTV发病率最高（52.50%），东区最低（37.71%）。CTV在植株中的空间分布表明，嫩枝中的病毒浓度最高，其次是叶片组织，而茎中的病毒浓度相对较低。在所有柑橘种植园中均观察到蚜虫大量滋生，南区最多，东区最少。锈线橘蚜是主要的蚜虫种类，其次是橘二叉蚜，不过蚜属种群数量明显较少。蚜虫滋生与CTV发病率呈显著正相关。
