Singh Amit Kumar, Meetei Ng Tombisana, Singh Brijesh Kumar, Mandal Nirmal
Department of Agricultural Biotechnology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252 India.
College of Horticulture and Forestry, Central Agriculture University, Pasighat, Arunachal Pradesh India.
Virusdisease. 2017 Dec;28(4):401-407. doi: 10.1007/s13337-017-0411-7. Epub 2017 Dec 5.
Khasi mandarin is one of the most remunerative crop in North Eastern Hills region of India playing a very critical role in the socio-economic upliftment of the people. The orchards of this region were found to express the typical symptoms of citrus tristeza virus (CTV). An extensive survey of the Khasi mandarin orchard for six North Eastern states of India namely Arunachal Pradesh, Meghalaya, Assam, Nagaland, Sikkim and Tripura, was carried out to establish the identity of these viruses using ELISA and PCR/RT-PCR techniques. Out of 300 Khasi mandarin tree samples collected from the six states, 172 were found to be positive for CTV infection by DAS-ELISA indicating 57.33% overall CTV disease incidence. Results revealed presence of CTV in all the surveyed states showing a maximum incidence of 66.00% in Arunachal Pradesh followed by 62.00% in Assam, 60.00% in Meghalaya and Nagaland, 54.00% in Sikkim and 42.33% in Tripura. Higher CTV concentration was recorded in the age group > 15 years (69.09%) followed by 10-15 (57%) and 5-10 years (43.33%). However, this study, to the best of our knowledge, is the first report for the detection of CTV in Khasi mandarin from Arunachal Pradesh, Nagaland and Tripura and also the first authentic survey of overall disease incidence of CTV Khasi mandarins from the six major mandarin growing North Eastern states of India.
卡西柑是印度东北部山区最具经济效益的作物之一，在当地人民的社会经济发展中发挥着至关重要的作用。该地区的果园被发现表现出柑橘衰退病毒（CTV）的典型症状。为了利用酶联免疫吸附测定（ELISA）和聚合酶链反应/逆转录聚合酶链反应（PCR/RT-PCR）技术确定这些病毒的特性，对印度东北部六个邦（即阿鲁纳恰尔邦、梅加拉亚邦、阿萨姆邦、那加兰邦、锡金邦和特里普拉邦）的卡西柑果园进行了广泛调查。从这六个邦采集的300份卡西柑树样本中，有172份通过双抗夹心ELISA检测出CTV感染呈阳性，表明CTV疾病总体发病率为57.33%。结果显示，所有被调查的邦都存在CTV，阿鲁纳恰尔邦的发病率最高，为66.00%，其次是阿萨姆邦的62.00%、梅加拉亚邦和那加兰邦的60.00%、锡金邦的54.00%以及特里普拉邦的42.33%。在年龄大于15岁的组中记录到更高的CTV浓度（69.09%），其次是10 - 15岁组（57%）和5 - 10岁组（43.33%）。然而，据我们所知，本研究是首次报道在阿鲁纳恰尔邦、那加兰邦和特里普拉邦的卡西柑中检测到CTV，也是对印度东北部六个主要柑橘种植邦的卡西柑CTV总体疾病发病率的首次权威调查。
