Liquid chromatography of clindamycin 2-phosphate on triethylaminoethyl cellulose.

The separation of clindamycin 2-phosphate from clindamycin 3-phosphate, clindamycin 4-phosphate, clindamycin B 2-phosphate, and lincomycin 2-phosphate was achieved by liquid chromatography on triethylaminoethyl cellulose using a 254-nm monitor. The compounds have low molar absorptivities at 254 nm (smaller than 17), and UV detection is made possible by the high capacity support triethylaminoethyl cellulose. Linear peak height response versus concentration allows rapid quantitation of clindamycin 2-phosphate.